Microprocessor UV-VIS Double Beam Spectrophotometer


  • Medicine/Pharmaceutical Industry
  •  Environment Monitoring
  •  Commodity Inspection
  •  Food Inspection
  •  Agricultural Chemistry
  •  Teaching in Colleges & Universities
  •  Metallurgy
  •  Geology
  •  Machine Manufacturing
  •  Petrochemical Industries
  •  Water and Waste water Labs
  •  Food and beverages Labs

Double Beam UV/VIS Spectrophotometer

spectrophotometer are a d vanced double beam optical system. Double beam optical structure can inhibit the drift, Suitable for long tim e te s t. with 0.5/1/1.5/2/4/5nm Variable bandwidth, They are suitable for research, biochemical and pharmaceutica l la b applications.



Wavelength Range 190-1100nm
Spectral Bandwidth 0.5/1/1.5/2/4/5nm Variable
Optical System Double Beam, Blazed Holographic Grating (1200 lines/mm)
Wavelength Accuracy ±0.5nm
Wavelength Repeatability ≤0.2nm
Wavelength Setting Auto, Resolution 0.1nm
Photometric Range 0~200%T,-4~4A,0~9999C
Photometric Accuracy ±0.002 A (0~0.5A), ±0.003A (0.5~1A), ±0.3%T (0~100%T)
Photometric Repeatability ≤0.001 A (0~0.5A), ≤0.002A (0.5~1A), ≤0.2%T (0~100%T)
Stray Light ≤0.05%T(220/360nm)
Scan Speed High, Medium, Low. Max.2000nm/minute
Baseline Flatness ±0.001A
Stability ±0.001A/h (500nm,0A)
Noise ≤0.2%T/3min (250/500nm,0%T); ≤0.3%T/3min (250/500nm,100%T)
Sample Compartment 10mm Pathlength Cuvette
Detector Silicon Photodiode
Lamps Tungsten Lamp & Deuterium Lamp (Pre-aligned)
Display Graphic LCD (320*240 Dots)
Keypad 30-key Alphanumeric Membrance Keypad
Output Port USB Port
Printer Mini Serial Printer; PC Printer
PC Software PC Scanning Software
Power Requirements AC 90-250V, 50/60Hz
Dimension 635x515x255mm
Weight 26kg



Double beam ensure low drift, low noise and Low stray light
High speed MCU, high precision AD, large storage capacity
Large LCD display (320*240 Dots)
1.0nm or variable Bandwidth meet Pharmacopoeia
Data and Curve can be stored in real-time
Online software upgrade capability
Lamps can be turned on/off individually
Easy to change Pri-aligned lamps


Quantitative(Standard Curve)
WL Scan(Spectrum Scan)
Time Scan(Kinetics)
DNA/Protein Test
Multi-WL Test
System Utility


Basic Mode:

To measure the Absorbance and transmittance


1. Coefficient Method
2. Standard Curve Up to 10 Standard sample may be used to establish a curve. Four methods for fitting a curve through the calibration points :
Linear fit. Linear fit through zero, Square fit and cubic fit.

DNA/Protein Test:

Concentration and DNA purity are quickly and easily calculated: Absorbance  rations: 260 nm / 280 nm with optional subtracted absorbance at 320 nm. DNA
concentration = 62.9XA260-36.0XA280 Protein concentration = 1552xA260-
757.3xA 280

Wavelength Scan:

1. The wavelength scan intervals are 0.1,0.2,0.5,1,2,5 nm
2. High, Medium and low scan speed are available. They vary from 100 to 3600 nm/min
3. Wavelength are scanned from high to low so that the instrument waits at high WL. And it minimizes the degradation of UV sensitive samples.


Abs vs time graphs is displayed on the screen in real time wait time and measurement time up to 12 hours may be entered with time interval of 0.5,1,2,5,10,30 seconds and one min. Post-run manipulation includes re-scalling, curve tracking and selection of the part of the curve required for rate calculation. Rate is calculated using a linear regression algorithm before multiplying be the entered factor.


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